The cees dekker Diaries
The cees dekker Diaries
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Making use of single-molecule optical and AFM imaging and pressure-spectroscopy tactics, we look for to unravel the mechanistic aspects of the composition and dynamics of chromosomes. Our investigation interests include:
It had been only in the nineties though, when nanotechnology had Highly developed into a amount in which one molecules might be manipulated, that the 1st experimental devices commenced to appear.
Multicolor imaging with the bacterial nucleoid and cell division proteins with blue, orange, and close to-infrared fluorescent proteins
A rhythmically pulsing leaf-spring DNA-origami nanoengine that drives a passive follower Mathias Centola
This yielded a breakthrough. Inside of only some months, we have been able to evaluate transportation by way of somebody solitary-walled carbon nanotube. Depending on small-temperature experiments by an excellent PhD university student in my group, Sander Tans (now a valued colleague and professor at Delft College of Technologies by having an active exploration lab at AMOLF in Amsterdam), we showed that carbon nanotubes have been legitimate quantum wires with really very long (micrometres) electronic coherence lengths — confirming theoretical predictions of metallic behaviour.
Our investigate ranges within the biophysics of DNA to artificial cells and further than. You should take a look under with the spectrum of our recent assignments.
We use nanofabricated microfluidic constructions to pattern spatial boundaries for germs and biomimetic vesicles with reconstituted protein networks and this evaluate the elemental job of spatial confinement within the molecular and mobile stage. Our assignments include things like the next:
Dynamic ParB–DNA interactions initiate and retain a partition condensate for bacterial chromosome segregation
In 1993, being a youthful and eager associate professor ceesdekker.net at Delft University of Technological know-how, I had at first centered the investigate efforts of my group on measuring the electrical conductance of an individual conducting-polymer molecule. Within just two years, we had developed a device where a single wire of a phthalocyaninepolysiloxane polymer was connected among two carefully spaced metallic nanoelectrodes.
Resolving the phase measurement in condensin-driven DNA loop extrusion identifies ATP binding since the stage-producing method
FtsZ treadmilling is important for Z-ring condensation and septal constriction initiation in bacterial mobile division
Afterwards we spoke once again briefly mainly because Dekker felt that our dialogue of his faith swiftly grew to become purely philosophical, While In addition it concretely helps him in his function.
Allow’s very first seem back again at this time when you decided to shift your concentrate from superconductivity and nanotubes to biology at the end of the nineties.
The biophysicist from Delft – who is Just about the most cited scientists in the Netherlands For some time – can take his accountability to contribute to the rationalization and popularisation of his operate on the border of physics and biology incredibly seriously and consistently seems within the media; Otherwise to look at his perform, then about the relationship involving science and his Christian faith.